January 26, 2019

El cultivo de protoplastos en cítricos y su potencial para el mejoramiento genético. Article (PDF Available) · July with 1, Reads. Export this citation. Results 80 – 90 Download Citation on ResearchGate | On Jan 1, , R.R. Rodríguez and others published Aislamiento y cultivo de protoplastos en maracuyá. Universidad de León – Grado en Biotecnología – Tercero Biotecnología – Biotecnología vegetal – Tema 9. Cultivo de protoplastos e hibridación somática.

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Many wild Solanum species are regarded as important sources for disease resistance and tolerance to many abiotic stresses Hawkes, but their use in potato breeding is limited due to poor crossability and sterility of interspecific hybrids.

In this study, confirmation of fusion-derived regenerants by the protoplashos of RAPD markers represented an effective system for the detection of somatic hybrids from S.

In Vitro Cell Dev. This study shows that somatic hybrid potato plants can be obtained by the fusion method presented.

Under these conditions, calli hybrids follow the developmental pathway of the morphogenic parents. When colonies had developed, alginate beads were despolimeryzed using 20 mM sodium citrate solution in sorbitol 0,3 M, pH 7,4 and xe shaking.


Tema 9. Cultivo de protoplastos e hibridación somática

Plant Cell Rep protoplastox Improvements in regeneration from protoplasts of potato and studies on chromosome stability. It has been suggested that protoplasts are more prone to chromosome instability because they undergo a long period of greater stress during initial stages of cell division and dedifferentiation Pijnaker and Sree Ramulu ; Carrasco et al.

Protoplsatos of somatic hybrids of potato by use of RAPD markers and isozyme analysis. Due to that, fused and unfused protoplasts were cultured together.

Tema 9. Cultivo de protoplastos e hibridación somática – Tercero Biotecnología. – Stuvia

Non-transgenic applications of plant tissue culture in potato. To select hybrids, regeneration of in vitro plants was subjected to RAPD analysis. This technique is a tool for somatic hybrid characterization Baird et al. Potato, Solanum tuberosum L.

Somatoclonal variation in potato: Plant Cell Tissue Organ Cult. Somaclonal variation in potato.

Nineteen somatic hybrid plants were obtained from fusion experiments using PEG in an effort to combine elite traits from both parents. Ruiz de Galarreta y E. A rapid calli proliferation was observed and most of them showed purple-colored cells Fig.

Protoplast fusion, culture and regeneration Prior to fusion, protoplasts from S. Preview 1 out of 20 pages. Earn hundreds of dollars each month by selling your written material to your fellow students. Protoplasts fusion and plant regeneration.


Cultivo y fusion de protoplastos [1990]

Chromosome observations indicated that all hybrids were aneuploids. These results showed that all hybrids were aneuploids Fig. Somatic hybridization in the genus Solanum: What do you want to do? The osmolality of this medium was fixed at mOsm. Root tips were stained by lacto-propionic orcein. Production and analysis of intraspecific somatic hybrids of potato Solanum tuberosum L.

Analysis of chromatin, nuclear DNA and organelle composition in somatic protoplasts between Solanum tuberosum and Solanum sanctae-rosae. Somatic hybrids obtained by protoplast fusion between Solanum tuberosum L.

Then the suspension was centrifugated at rpm during ten minutes. Plant material and protoplast isolation. For the obtained somatic hybrid plants, the chromosome number varied from Table 2. Tissue culture procedures to produce somatic hybrids and the application of molecular biological tools for their analysis are well established.